Do-Si-Do #22 F2 - GSC x Face Off OG: A Comprehensive Strain Guide

Overview and Naming

Do-Si-Do #22 F2 – GSC x Face Off OG – is a modern heir to two of the most influential West Coast lines of the last decade. It blends the dessert-tier sweetness of the Girl Scout Cookies family with the narcotic, pine-fuel backbone of Face Off OG. The #22 designation refers to a prized selection celebrated for color, resin density, and an unmistakable ‘cookie gas’ profile.

The F2 label signals a second filial generation, where breeders re-crossed F1 offspring to unlock more variation and access hidden traits. For growers and connoisseurs, that means richer phenotypic diversity, more opportunities to select elite keepers, and a wider palette of terpene expressions rooted in the same iconic parents.

In practical terms, Do-Si-Do #22 F2 is a resin-forward cultivar often prized by extractors and hashmakers. It’s also a high-THC flower that regularly tests above 22% total THC, placing it squarely in the top quartile of potency among dispensary offerings in regulated U.S. markets.

History and Breeding Context

Do-Si-Dos emerged from Archive Seed Bank’s work pairing an OGKB (a notorious, resin-coated GSC phenotype) with Face Off OG BX1. The result set a new standard for cookie-kush hybrids: dense, purple-kissed flowers, mint-cookie sweetness, and an unmistakable OG exhale. The #22 cut rose within that universe as a standout for color, structure, and solventless-friendly resin.

As the cultivar gained notoriety, breeders moved into F2 work to stabilize specific traits while allowing recombination to throw novel winners. F2 generations typically show wider phenotypic variance than S1s or tightly inbred lines. For growers, that variability is strategic: it increases the odds of finding a plant that excels in their exact environment and production goals.

By the late 2010s and early 2020s, Do-Si-Do derivatives were among the most entered cultivars in extraction competitions. Reports from craft producers consistently placed Do-Si-Do-derived washes in the 4–6%+ fresh frozen range, a strong indicator of trichome head size and cuticle integrity. The #22 F2 line maintains that hashmaker appeal while delivering a flavor-driven, boutique flower experience.

Genetic Lineage and Pheno #22 Explained

The parentage here is Girl Scout Cookies (via the OGKB expression) crossed with Face Off OG. OGKB contributed the cookie-dough sweetness, mint-chocolate nuance, and the dense, glazed-truffle look. Face Off OG contributes the kush spine: pine, fuel, and a heavy, behind-the-eyes relaxation that many users describe as ‘face melting.’

Pheno #22 is shorthand for a breeder or grower’s selected plant that met strict performance criteria. In this case, #22 is known for its high calyx-to-leaf ratio, striking anthocyanin expression, and a ‘sweet-gas-lavender’ terpene arc. It also tends to exhibit more pronounced lateral branching and slightly tighter internodal spacing, favorable for SCROG.

F2 breeding increases the chance of encountering phenotypes that lean strongly to either side of the pedigree or land dead-center. Growers can expect three broad buckets: cookie-leaning dessert aromatics, OG-leaning gas and pine, and a blended ‘cookie-gas’ set that many consider the holy grail. Within those groups, resin type, wash yield, and color expression will vary, rewarding a thorough phenohunt.

Appearance and Structure

Do-Si-Do #22 F2 flowers are typically dense and golf-ball to egg-shaped, with swollen calyxes that stack into compact colas. The coloration ranges from mint green to deep olive, often with lavender to plum highlights when night temperatures drop below 65°F (18°C). Fiery orange to rust pistils weave through a sheet of frosty trichomes.

Trichome coverage is heavy, frequently described as ‘sugar-coated’ or ‘iced.’ Under a loupe, capitate-stalked gland heads appear uniform and bulbous, with a notable proportion in the 90–120 µm range favored by hash makers. In bright light, the resin gives the buds a silvery sheen that persists even after trimming.

The leaf-to-calyx ratio is favorable for post-harvest, reducing trim time relative to fluffier cultivars. Internodal spacing averages 2–4 inches (5–10 cm), lending itself to tight canopies and efficient light utilization. Plants tend to finish with a photogenic bag appeal that translates well to both retail jars and macro photography.

Aroma and Bouquet

On first crack, expect a layered perfume of sweet dough, powdered sugar, and vanilla-mint overlaid with pine and faint diesel. As the buds warm, the profile deepens into lavender, fennel, and earthy cocoa with a citrus twist from limonene. Many users describe a ‘thin mint dipped in gas’ impression that’s both nostalgic and modern.

Terpene volatility is notable: top notes bloom quickly, while base notes linger in the room long after the jar is sealed. In properly cured batches with 1.8–2.5% total terpene content, the room-filling aroma is evident within seconds. OG-leaning phenotypes push more fuel and pine, while cookie-leaners emphasize bakery sweetness and floral spice.

Grinding the flower releases anise, violet, and a peppery snap from beta-caryophyllene. Myrcene and linalool contribute a soft, herbal undercurrent that reads as relaxing and slightly perfumed. The bouquet’s complexity is a hallmark of the #22 selection and a primary reason it remains a connoisseur favorite.

Flavor and Mouthfeel

The inhale opens with sweet shortbread and vanilla, followed by a cooling mint-herbal lift. Mid-palate brings pine resin, lavender, and a faint pistachio-nutty accent that echoes the cookie lineage. On exhale, diesel-kush and cracked pepper add depth, leaving a long, slightly floral finish.

When vaporized at 380–400°F (193–204°C), bright citrus-lavender and creamy cookie notes dominate, with less fuel on the tail. Combustion leans the flavor more OG, revealing pine, petrol, and black pepper atop a confectioner’s sugar base. The smoke is typically smooth and thick, with a mouth-coating resin feel.

Users consistently report that flavor persists through multiple pulls, with minimal drop-off by the third or fourth draw. In blind tastings, Do-Si-Do #22 F2 often scores highly for aftertaste persistence and perceived complexity. That longevity is a strong indicator of robust terpene retention through harvest and cure.

Cannabinoid Profile and Potency

Lab-tested batches of Do-Si-Do progeny frequently register total THC in the 22–30% range, with rare elite cuts exceeding 30% under optimized conditions. The #22 F2 line commonly lands between 23–28% THC for well-grown indoor flower. CBD is typically low at 0.1–0.6%, while total cannabinoids often reach 25–33%.

Minor cannabinoids add nuance. CBG is commonly detected in the 0.3–1.2% range, and THCV appears in trace amounts (often <0.2%). Total acidic cannabinoids (THCA-dominant) can exceed 27% in top-tier samples, translating to a potent decarboxylated THC payload after activation.

Potency perception aligns with the numbers. In consumer surveys across multiple markets, Do-Si-Do-type cultivars are regularly rated ‘strong’ or ‘very strong’ by >70% of respondents. The curve skews toward evening use due to intensity, despite a euphoric, talkative onset at lower doses.

Terpene Profile and Minor Aromatics

Total terpene content for Do-Si-Do #22 F2 typically falls between 1.5–3.0% by dry weight when grown and cured optimally. Beta-caryophyllene (0.4–0.9%), limonene (0.3–0.8%), and linalool (0.1–0.4%) are the usual anchors. Myrcene (0.2–0.6%) and humulene (0.15–0.35%) commonly round out the top five.

Secondary and trace contributors include ocimene (0.05–0.20%), nerolidol (0.03–0.12%), and terpinolene in very small amounts, which can subtly brighten the nose. This combination drives the confectionary-floral top with a spicy, woody base and a citrus lift. The caryophyllene–linalool pairing, in particular, correlates with the cultivar’s relaxing character.

From an extraction standpoint, the cultivar’s terpene balance translates well to both solvent and solventless formats. Ice water hash makers report good mechanical separation due to robust, intact gland heads. Live rosin often retains the vanilla-mint and lavender layers with a diesel backnote, testing 4–8% total terpene content in fresh-pressed material.

Experiential Effects and Use Patterns

The onset is typically fast within 2–5 minutes when inhaled, with an initial mood lift and gentle cerebral clarity. A warm body wave follows, settling into shoulders and the back of the neck over 10–20 minutes. Users often describe a subtle pressure behind the eyes and a calm, grounded focus.

At moderate doses, expect a balanced euphoria and relaxation that remains functional for 45–90 minutes. Music, cooking, and small-group socializing pair well during this window. As the session deepens or doses increase, sedation becomes more prominent, and couchlock is possible.

Self-reported effects commonly include uplifted (60–75% of users), relaxed (70–85%), and euphoric (55–70%). Dry mouth and dry eyes are the most frequent side effects, while transient dizziness appears in a small minority at high doses. Paranoia is uncommon but can occur in THC-sensitive individuals, particularly with rapid, repeated inhalation.

For daily rhythms, many reserve Do-Si-Do #22 F2 for late afternoon through evening. Creative tasks and reflective conversations can shine in the first half-hour, followed by a comfortable descent. For some, the cultivar doubles as a nightcap due to its body-soothing finish.

Potential Medical Applications

While not a substitute for medical advice, the profile of Do-Si-Do #22 F2 suggests several potential therapeutic use-cases. The high THC content, combined with beta-caryophyllene and linalool, may support analgesic and anxiolytic effects in certain individuals. Users commonly report relief in categories such as stress, generalized body aches, and difficulty falling asleep.

Preclinical and clinical literature has associated THC with analgesia and muscle relaxation, which aligns with user reports of reduced tension. Myrcene and linalool are frequently linked to calming and sedative properties, which may explain the cultivar’s night-time utility at higher doses. Appetite stimulation is also commonly observed, beneficial for those navigating reduced appetite.

For sensitive users, lower dose strategies (one or two short inhalations or a 2.5–5 mg edible equivalent) may maximize benefits while minimizing anxiety. Tracking response over multiple sessions is advisable, as tolerance and individual endocannabinoid differences can change outcomes. As always, patients should consult a qualified clinician, especially when combining cannabis with other medications.

Cultivation Guide: Indoors and Outdoors

Do-Si-Do #22 F2 is moderately vigorous with a 1.5–2.0x stretch after flip. Flowering time averages 60–70 days indoors, with many keepers peaking at day 63–67 for optimal terpene and resin. Outdoors, harvest typically comes in late September to early October in temperate climates.

Yields are respectable to high with proper canopy management. Indoor growers commonly achieve 1.5–2.5 oz/ft² (450–750 g/m²) under high-efficiency LEDs at 800–1,000 µmol/m²/s PPFD. Outdoor yields range from 500–1,000 g per plant in 20–50 gallon containers, with training and climate playing large roles.

Vegetative growth responds well to topping at the fourth or fifth node and lateral training. SCROG and manifold techniques create even canopies and increase light penetration to the middle sites. Lollipopping lower growth in week 2–3 of flower helps divert energy to dominant colas and reduces humidity pockets.

Environmental targets that perform well include day temps of 75–82°F (24–28°C) and night temps of 62–72°F (17–22°C). Relative humidity at 60–70% in veg, 45–55% in early to mid flower, and 40–45% in late flower helps balance VPD at 1.0–1.3 kPa. Lowering nights to 58–65°F (14–18°C) in the finish can coax deeper purples in anthocyanin-positive phenos.

Nutrient demand skews moderate on nitrogen and heavier on P/K from weeks 4–7 of flower. Many growers report success with EC 1.6–2.0 mS/cm in mid flower and up to 2.2 mS/cm for high-PPFD, CO₂-enriched rooms. Maintain root-zone pH at 5.8–6.2 (soilless) or 6.2–6.8 (soil) and ensure ample calcium and magnesium to avoid tip burn and interveinal chlorosis.

Airflow is critical due to tight buds; two canopy fans per 4×4 ft (1.2×1.2 m) area and robust exhaust help prevent powdery mildew. This line can be moderately susceptible to PM and botrytis in dense canopies, especially above 55% RH late in flower. Defoliation should be conservative but targeted to open interior microclimates.

In CO₂-enriched environments (1,000–1,200 ppm), raise PPFD to 1,000–1,200 µmol/m²/s and monitor leaf temps with an IR gun, targeting 84–86°F (29–30°C) leaf surface. Keep DLI near 45–55 mol/m²/day in flower for optimal resin and terpene expression. Excessive heat or DLI can mute linalool and limonene, reducing the cultivar’s signature nose.

Outdoors, the plant appreciates full sun and a well-draining, living soil with high organic matter. Preventive IPM is wise from pre-flower onward, with weekly scouting for caterpillars and mites. Mulch and consistent irrigation mitigate heat stress and maintain steady nutrient uptake.

For irrigation, aim for 10–20% runoff in soilless systems and allow moderate dry-backs to encourage root vigor. In living soil beds, pulse watering to field capacity and avoid severe dry-down to protect soil biology. Enzymes or beneficial microbes can support nutrient cycling and root health through the mid-flower demand spike.

Harvest, Post-Processing, and Extraction

Peak harvest typically occurs when 10–15% of trichomes have turned amber with the remainder cloudy, often around day 63–67. Harvesting earlier (all cloudy, minimal amber) preserves brighter mint-citrus top notes, while a later window deepens gas and pepper. Many growers split-harvest top colas first and lower sites 3–5 days later for uniform ripeness.

Drying at 60–62°F (15.5–16.5°C) and 55–60% RH for 10–14 days helps lock in the pastry-floral aromatics. A slow dry reduces chlorophyll edge and protects linalool and ocimene, which are more volatile. Curing in airtight containers at 58–62% RH for 3–6 weeks further integrates the flavor layers.

For solventless, fresh frozen material washed within 24 hours of harvest often returns 4–6%+ (fresh frozen to hash) in resin-rich phenos, with some reports higher under optimized conditions. Good selections press 70–80%+ from hash to rosin, yielding terp-saturated live rosin. For flower rosin, 20–28% returns are not uncommon with properly dried and cured material.

Solvent extraction captures the full gas spectrum and can test above 75% total cannabinoids with 6–10% terpenes in live resin. Hydrocarbons tend to emphasize the OG backbone, while rosin spotlights the cookie-lavender. Post-processing into badder or cold-cured rosin preserves the confectionery profile prized by consumers.

Phenohunting Notes and Selection Tips

In F2 populations, plan to run a larger number of seeds to see the full spread—12–24 plants is a practical baseline for hobbyists, 48–96+ for pros. Track vigor, node spacing, lateral branching, and pre-flower aroma to identify promising keepers early. Many elite #22-esque phenos show a balanced ‘dessert gas’ pre-flower stem rub with anise and lavender.

Resin quality is the decisive criterion for many. Use a jeweler’s loupe to assess gland head size and stem thickness around week 7; look for plump, uniform heads that detach cleanly during a light rub. Test-wash small samples if possible, and log yields alongside sensory outcomes.

For flower production, prioritize phenos with a high calyx-to-leaf ratio and even budset from top to mid-canopy. Color is a bonus but should not trump structure and resin traits. Keepers often exhibit minimal foxtailing under high light and hold terps well through an